AgarSqueezer is a microscope slide chamber equipped with a molded agar-based compression system. Use it to apply an instant & homogeneous compression on your cells, and study their response to short and long-term confinement.



 

Applications of AgarSqueezer

Agarsqueezer has been successfully used to confine adherent and non-adherent cells, including:
> Human: primary T-lymphocytes, TF1 & ML2 leukemic cells, HS27A fibroblasts, MCF10A breast cells, MDA-MB-231 breast cancer cells, U-2 OS osteosarcoma cells, PC-3 & DU 145 prostate cancer cells, HT29 & HCT116 colorectal adenocarcinoma cells and HT1080 fibrosarcoma cells
> Murine: megakaryocytes, osteocyte-like cells MLO-Y4, primary muscle cells & primary dendritic cells
> Plant: cells from Arabidopsis roots
> 3D cell cultures: mice gastruloïds

Compatible assays: 
> Live imaging*: cell viability, proliferation, migration, morphology, nuclear deformability, cytoskeleton reorganization, cell tracking, etc
> Standard molecular analysis (immunostaining, western blot, qPCR, flow cytometry, etc): changes in gene/protein expression, proliferation, morphological analyses, etc 

*Cells can be added with fluorescent probes or stimulated with drugs during confinement.

Imaging modes: 
Inverted microscopes equipped with a standard adjustable petri dish holder, with any imaging mode: phase-contrast, epifluorescence, confocal, etc. 

 

Kit content of AgarSqueezer

• 1x Agarsqueezer compression device
• 1x  16G flat cut needle to make holes in the agar gel, facilitating diffusion of culture medium or drugs during the experiment
• 1x 20G flat cut needle (same as above)
• (Optional) 1x device holder (102.5 mm x 143.5 mm), to place and image up to 2 AgarSqueezers in live imaging chambers (Okolab or equivalent). 

In addition to basic kit contents, we recommend using a silicium wafer to mold your confining pillars in agarose. We provide four different wafers to mold pillars of various heights. Choose among our four pillar heights available depending on your desired application and add silicium wafer(s) to your order here. 



Results:

 Compression of immature TF1-GFP hematopoietic cells using AgarSqueezer

 

Arabidopsis root cells confined using AgarSqueezer

Compression of mouse primary myoblasts using AgarSqueezer

Observation of vesicles at septum during cell division of mutant E. coli (W3110 tolR -  Palmcherry),
in LB 1/2 medium, using AgarSqueezer.

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