DESCRIPTION: Highly purified type II collagen (treated with 3M guanidine, DEAE-cellulose and Na₂ HPO₄ )
APPLICATION: Ideal as an ELISA antigen for detecting antibodies specific to human type II collagen in sera from experimental animals and humans, such as collagen-induced arthritis (CIA) models and patients with arthritis or other diseases, as well as normal controls.
QUANTITY: 1 mL
FORM: 0.5 mg/mL solution in 0.05M acetic acid
SOURCE: Human sternal cartilage
MOLECULAR WEIGHT: 300 Kd
PURITY: >99% by SDS-PAGE, free of pepsin and proteoglycans
USAGE: It is recommended to dilute this product with chilled Collagen Dilution Buffer, (catalog # 9003) provided with the ELISA grade collagen, to prevent the aggregation of collagen molecules. For coating, take aliquots and dilute it with the Collagen Dilution Buffer just before use. Do not keep diluted solutions. The optimum concentration of collagen to coat ELISA plates is 5μg/mL generally, but may vary based on the type of plate used for different purposes such as coating cell culture plates.
STORAGE TEMPERATURE: -20°C in the dark. This product is stable in acidic conditions at 4°C for several weeks and at -20°C for one year as supplied, but will gradually form polymeric collagen and degrade under neutral conditions.
STABILITY: 1 year
REFERENCES: Trelstad R. et al. Biochemistry 9: 4993-4998 (1970)
Miller EJ. Biochemistry 10: 1652-1659 (1971)
Terato K. et al. Arthritis Rheumatism 33: 1493-1500 (1990)
Terato K. et al. Clin. Immunol. Immunopathol. 79: 142-154 (1996)