特色產品：
綿羊抗大鼠腎小球基底膜血清（PTX-001AGBM）
Sheep Anti Rat Glomerular Basement Membrane (GBM) Serum
--抗GBM型腎炎模型誘導
|
產品名稱 |
Sheep Anti Rat Glomerular Basement Membrane (GBM) Serum |
|
貨號 |
PTX-001AGBM |
|
規格 |
25ml |
|
保存 |
-20℃ |
|
說明 |
產品經56℃熱處理30min，請避免反復凍融。解凍后會有輕微沉淀，注射前3000-5000rmp 離心30min。
注：只做過大鼠炎癥，沒有做過小鼠驗證。 |
應用說明：(實驗前請仔細閱讀實驗流程)
每瓶血清含有足夠的抗體用于誘導至少25只大鼠*（175-200 g體重）被動抗GBM腎炎模型。
免疫抗GMB血清：麻醉大鼠，尾靜脈注射抗GMB血清超過15秒，疾病的產生依賴于免疫劑量，依照免疫劑量完全打入體內很關鍵（推薦用量：0.4-0.5ml/100g體重）。由于不同批次抗血清差異，大鼠來源，以及實驗要求的不同，我們建議進行劑量-反應研究，具體的免疫劑量和疾病的嚴重程度，需要根據每個不同批次血清和產生疾病的嚴重程度，選擇合適的免疫劑量。
腎病研究：
1）異源性疾病：免疫抗GBM (anti GBM)3-5天后，免疫熒光檢測顯示綿羊IgG在腎小球基底膜內呈線狀沉積。大鼠c3也呈線性分布，24小時后出現蛋白尿。
2）自身疾病：免疫抗GBM抗體8-10天后，檢測大鼠蛋白尿增加，同時大鼠腎小球IgG(自身抗體) 呈線狀沉積，自體疾病變得明顯。（自體）免疫組化呈線性模式的IgG（圖1）。到3周時，蛋白尿有望達到200毫克/24小時，腎小球病變（圖2）明顯，隨著時間的推移，嚴重程度增加，導致腎小球硬化。
圖1.免疫熒光檢測顯示免疫抗GBM3周后腎小球毛細血管壁大鼠IgG免疫沉積的。
圖2.免疫組化染色顯示免疫抗GBM 3周后新月體形成、系膜細胞增生、毛細血管變形。
相關產品：
|
貨號 |
產品名稱 |
規格 |
|
腎炎模型 |
|
PTX-001AGBM |
Sheep Anti-Rat GBM Serum |
25ml |
|
PTX-002S |
Sheep Anti-Rat Fx1A Serum |
25ml |
|
PTX-003S |
Sheep Anti-Rat Thy-1 Serum |
20ml |
|
PTX-000S |
Sheep Non-Immune Serum |
25ml |
|
組織產品（動物疾病模型組織、切片、血漿、尿液） |
|
PTX-001 |
Anti-GBM (Immune-mediated Glomerulonephritis) |
- |
|
PTX-002 |
Anti-Fx1A (Heymann Nephritis, Membranous Nephritis) |
- |
|
PTX-003. |
Anti-Thy-1 (Mesangial Proliferative Glomerulonephritis) |
- |
|
PTX-004 |
Diabetic nephropathy (streptozotocin- Type I Diabetes) |
- |
|
PTX-005 |
Control (Normal) Kidney |
- |
|
PTX-006 |
Sheep Kidney Section(anti-GBM and Fx1A membranous nephropathy |
- |
|
細胞 |
|
CRGMes-1 |
Rat Glomerular Mesangial Cells |
1x106 |
|
CRGEp-1 |
Rat Glomerular Epithelial Cells |
1x106 |
|
CBGEn-1 |
Bovine Glomerular Endothelial Cells |
1x106 |
|
CMUB-1 |
Mouse Ureteric Bud Cells |
1x106 |
|
CMMM-1 |
Mouse Metanephric Mesynchymal Cells |
1x106 |
|
CRMM-1 |
Rat Metanephric Mesynchymal Cells |
1x106 |
|
抗體 |
|
BS-001GBM |
FITC- Sheep Anti-Glomerular Basement Membrane IgG |
0.5ml |
|
BS-002ECM |
FITC- Sheep Anti-Extracellular Matrix (ECM) IgG |
0.5ml
|
參考文獻：
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